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Image Search Results
Journal: International Journal of Immunopathology and Pharmacology
Article Title: Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells
doi: 10.1177/0394632015617065
Figure Lengend Snippet: Influence of SY on LPS-induced iNOS and Arg-1 expression in BV2 microglia. BV2 microglia were treated with LPS (0.5 μg/mL) or LPS (0.5 μg/mL) plus SY (80 μg/mL) for 48 h. Protein levels of iNOS (M1) and Arg-1 (M2) were measured by immunocytochemistry staining (a), flow cytometry (b), and western blot assay (c). Quantitative results are expressed as mean ± SEM from three independent experiments with similar results (in triplicate/each experiment). *P <0.05, **P <0.01.
Article Snippet: For intracellular staining, BV2 microglia were stained for 20 min at RT in 0.3% saponin/1% BSA-PBS buffer with the following antibodies: PE-IL-10 and PE-IL-12 (eBioscience, USA),
Techniques: Expressing, Immunocytochemistry, Staining, Flow Cytometry, Western Blot